microreactor array slide  (Biodesign International Inc)

Journal: Scientific Reports

Article Title: Microreactor Array Device

doi: 10.1038/srep08736

Figure Lengend Snippet: (A) Top view schematic of device consisting of a rigid frame with fasteners clamping a transparent window and transparent sealing membrane onto an O-ring sealing around the perimeter of a microreactor array slide consisting of microreactors with functionalizing chemicals. There are inlet & outlet ports for reagent at the bottom and inlet & outlet ports for sealing liquid at the top. Features are not drawn to scale. (B) Section view schematic of same device illustrating the process of displacing excess reagent and sealing microreactors. After completely filling the microreactors, excess reagent is displaced from the slide by pressurized sealing liquid injected through a sealing liquid inlet port acting on a sealing membrane. As the sealing liquid spreads out across the sealing membrane it first displaces excess reagent though reagent inlet & outlet ports and then seals the microreactors into isolated chemical reaction containers. The schematics (A, B) were drawn by Peter Wiktor.

Article Snippet: Insert a microreactor array slide into the fill & seal device of (Biodesign Institute, ASU, Tempe AZ).

Techniques: Membrane, Injection, Isolation

Journal: Scientific Reports

Article Title: Microreactor Array Device

doi: 10.1038/srep08736

Figure Lengend Snippet: Section view schematic of alternate microreactor fill & seal device based on sealing microreactors using silicone rubber instead of a viscous sealing liquid. Features are not drawn to scale. The schematic was drawn by Peter Wiktor.

Article Snippet: Insert a microreactor array slide into the fill & seal device of (Biodesign Institute, ASU, Tempe AZ).

Techniques:

Journal: Scientific Reports

Article Title: Microreactor Array Device

doi: 10.1038/srep08736

Figure Lengend Snippet: Signal to background ratio of fluorescently labeled protein spots using five different microreactor sealing methods are shown. Signal is the average of the median pixel intensity of the protein spots from the subarrays in . Background is the average of the median pixel intensity of the adjacent spots. Proteins were expressed in situ in a microarray format using NAPPA chemistry.

Article Snippet: Insert a microreactor array slide into the fill & seal device of (Biodesign Institute, ASU, Tempe AZ).

Techniques: Labeling, In Situ, Microarray

Journal: Scientific Reports

Article Title: Microreactor Array Device

doi: 10.1038/srep08736

Figure Lengend Snippet: Signal to background ratio of anti-p53 antibody response in cancer patient blood serum detected on NAPPA microreactor array vs. standard NAPPA on flat microscope slides. The height of the bar for each dilution point is the average signal to background ratio of six spots on the arrays. Error bars indicate signal range for the six spots. Signal of each spot is the median pixel value of the spot. Background is the median signal of all of the spots on the array.

Article Snippet: Insert a microreactor array slide into the fill & seal device of (Biodesign Institute, ASU, Tempe AZ).

Techniques: Microscopy